Estimation of penicillin concentrations and penicillin metabolising enzyme activities by the lowry reaction.

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Estimation of penicillin concentrations and penicillin metabolising enzyme activities by the Lowry reaction.

Sir: As the present methods for determination of concentrations of penicillins, and activities of penicillin-metabolising enzymes, are not completely satisfactory we examined the possibility of using the LOWRY reaction with penicillins1) for this purpose. All penicillins tested reacted with LOWRY reagent. Table 1 shows that ampicillin, 6-aminopenicillanic acid (6-APA), carbenicillin and several...

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Elevating Serum Penicillin Concentrations

The rapid renal clearance of penicillin has made it difficult to maintain blood levels adequate to combat resistant organisms such as enterococci and many staphylococci. Diodrast (1), p-amino-hippurate (PAH) (2), benzoic acid (3), sodium benzoate (3, 4), and carinamide (5), have been found to block renal tubular excretion of penicillin with enhancement of blood levels. Of these compounds, only ...

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Isolation of a Penicillin Acylase Producing E.coli and Kinetic Characterization of the Whole Cell Enzyme Activity

Penicillin acylase (EC 3.5.1.11) has been a target of study for a long time because of its pivotal role in the deacylation of the penicillin into the 6- aminopenicillanic acid (6-APA) and the side-chain organic acids. This property of penicillin acylase has been exploited commercially for large scale production of 6-APA, which is the key intermediate in the manufacture of semi-synthetic penicil...

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The co-killing of penicillin sensitive and penicillin resistant bacteria at low concentrations of the antibiotic.

There are a number of reports that bacteria which have been made resistant to antibiotics may be rendered normally susceptible by contact with sensitive organisms, with extracts of such organisms, or with culture filtrates. Some of these reports are mutually contradictory in important respects, and the results have not been reproducible regularly by other workers. Voureka (1948) found that when...

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Penicillin-induced Jarisch-Herxheimer reaction.

OBJECTIVE To report a case of Jarisch-Herxheimer reaction (JHR) in a patient with presumed neurosyphilis and HIV. CASE SUMMARY A 45-year-old HIV-positive man (CD4+ count 450 cells/mm(3) and history of AIDS-defining illness) presented with JHR after an initial intravenous dose of penicillin G for presumed neurosyphilis. The patient described feeling cold with worsening headache and chills appr...

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ژورنال

عنوان ژورنال: The Journal of Antibiotics

سال: 1980

ISSN: 0021-8820,1881-1469

DOI: 10.7164/antibiotics.33.1386